dapt 10 um Search Results


dapi  (Thermo Fisher)
99
Thermo Fisher dapi
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
dapi - by Bioz Stars, 2026-05
99/100 stars
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dapt  (Tocris)
96
Tocris dapt
Dapt, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-05
96/100 stars
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dapt  (Selleck Chemicals)
96
Selleck Chemicals dapt
a-b, Schematic of experimental workflow and UMAP plot of isolated single cells from the indicated micromass organoid conditions (ref ). Fibroblast Monoculture: organoid containing fibroblasts only; Fibroblasts + ECs: organoids containing fibroblasts and ECs; Fibroblasts + ECs + <t>DAPT:</t> organoids containing fibroblasts and ECs treated with a NOTCH inhibitor. c, UMAP plot of cells from organoid culture shaded by level of fibrogenic gene signature score as calculated with UCell. d, Heatmap of selected genes grouped by experimental condition. e-f, RT-qPCR analysis of TGFB isoform and receptor gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without DAPT (10 uM) for 72 hours. g-h, Immunoblots <t>of</t> <t>TGFβ</t> isoforms and receptors with lysates from unstimulated or DLL4 stimulated fibroblasts (72h). i, RT-qPCR analysis of COL1A1 , COL3A1 and COL6A1 gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without TGFβ inhibitor (SB431542; 10 uM) or DAPT (10 uM) for 72h. j , ELISA quantification of fibroblast production of pro-collagen I alpha 1 over 24h after 5 days of treatment with siRNA (20nM) during or without DLL4 stimulation. k-l , RT-qPCR and ELISA quantification of POSTN (k) and COMP (l) after 72 hours of stimulation with recombinant TGFβ1 (10 ng ml−1) (left) or with DLL4 stimulation and siRNA targeting of TGFBR2 (right). For e,f,i each data point represents an independent cell line (n = 6) and for j-l each data point represents biological replicates (n = 3) from a single cell line and are representative of at least two independent experiments. Data are shown as mean +/- s.d. Statistical analysis was performed by a two-sided ratio paired t-test for e , f, i and unpaired two-sided Student’s t-test for j-l.
Dapt, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapt/product/Selleck Chemicals
Average 96 stars, based on 1 article reviews
dapt - by Bioz Stars, 2026-05
96/100 stars
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pbs  (Nikon)
99
Nikon pbs
a-b, Schematic of experimental workflow and UMAP plot of isolated single cells from the indicated micromass organoid conditions (ref ). Fibroblast Monoculture: organoid containing fibroblasts only; Fibroblasts + ECs: organoids containing fibroblasts and ECs; Fibroblasts + ECs + <t>DAPT:</t> organoids containing fibroblasts and ECs treated with a NOTCH inhibitor. c, UMAP plot of cells from organoid culture shaded by level of fibrogenic gene signature score as calculated with UCell. d, Heatmap of selected genes grouped by experimental condition. e-f, RT-qPCR analysis of TGFB isoform and receptor gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without DAPT (10 uM) for 72 hours. g-h, Immunoblots <t>of</t> <t>TGFβ</t> isoforms and receptors with lysates from unstimulated or DLL4 stimulated fibroblasts (72h). i, RT-qPCR analysis of COL1A1 , COL3A1 and COL6A1 gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without TGFβ inhibitor (SB431542; 10 uM) or DAPT (10 uM) for 72h. j , ELISA quantification of fibroblast production of pro-collagen I alpha 1 over 24h after 5 days of treatment with siRNA (20nM) during or without DLL4 stimulation. k-l , RT-qPCR and ELISA quantification of POSTN (k) and COMP (l) after 72 hours of stimulation with recombinant TGFβ1 (10 ng ml−1) (left) or with DLL4 stimulation and siRNA targeting of TGFBR2 (right). For e,f,i each data point represents an independent cell line (n = 6) and for j-l each data point represents biological replicates (n = 3) from a single cell line and are representative of at least two independent experiments. Data are shown as mean +/- s.d. Statistical analysis was performed by a two-sided ratio paired t-test for e , f, i and unpaired two-sided Student’s t-test for j-l.
Pbs, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs/product/Nikon
Average 99 stars, based on 1 article reviews
pbs - by Bioz Stars, 2026-05
99/100 stars
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dap3 nm 033657 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human death associated protein 3 DAP3 nuclear gene encoding mitochondrial protein transcript variant 1 mGFP tagged
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sumo 1 sumo1 nm 001005781 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human SMT3 suppressor of mif two 3 homolog 1 S cerevisiae SUMO1 transcript variant 2 Myc DDK tagged
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recombinant human dap3 protein  (Novus Biologicals)
N/A
The Recombinant Human DAP3 Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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recombinant human dapk3 zipk protein  (Novus Biologicals)
N/A
The Recombinant Human DAPK3 ZIPK Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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dap3 nm 004632 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human death associated protein 3 DAP3 nuclear gene encoding mitochondrial protein transcript variant 2 mGFP tagged
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human dap12 alexa fluor« 350 conjugated antibody  (R&D Systems)
N/A
The Human DAP12 Alexa Fluor« 350 conjugated Antibody from R D Systems is a mouse monoclonal antibody to DAP12 This antibody reacts with human The Human DAP12 Alexa Fluor« 350 conjugated Antibody has been validated
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human fyb adap slap130 alexa fluor« 594 conjugated antibody  (R&D Systems)
N/A
The Human FYB ADAP SLAP130 Alexa Fluor« 594 conjugated Antibody from R D Systems is a mouse monoclonal antibody to FYB ADAP SLAP130 This antibody reacts with human The Human FYB ADAP SLAP130 Alexa Fluor«
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Image Search Results


a-b, Schematic of experimental workflow and UMAP plot of isolated single cells from the indicated micromass organoid conditions (ref ). Fibroblast Monoculture: organoid containing fibroblasts only; Fibroblasts + ECs: organoids containing fibroblasts and ECs; Fibroblasts + ECs + DAPT: organoids containing fibroblasts and ECs treated with a NOTCH inhibitor. c, UMAP plot of cells from organoid culture shaded by level of fibrogenic gene signature score as calculated with UCell. d, Heatmap of selected genes grouped by experimental condition. e-f, RT-qPCR analysis of TGFB isoform and receptor gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without DAPT (10 uM) for 72 hours. g-h, Immunoblots of TGFβ isoforms and receptors with lysates from unstimulated or DLL4 stimulated fibroblasts (72h). i, RT-qPCR analysis of COL1A1 , COL3A1 and COL6A1 gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without TGFβ inhibitor (SB431542; 10 uM) or DAPT (10 uM) for 72h. j , ELISA quantification of fibroblast production of pro-collagen I alpha 1 over 24h after 5 days of treatment with siRNA (20nM) during or without DLL4 stimulation. k-l , RT-qPCR and ELISA quantification of POSTN (k) and COMP (l) after 72 hours of stimulation with recombinant TGFβ1 (10 ng ml−1) (left) or with DLL4 stimulation and siRNA targeting of TGFBR2 (right). For e,f,i each data point represents an independent cell line (n = 6) and for j-l each data point represents biological replicates (n = 3) from a single cell line and are representative of at least two independent experiments. Data are shown as mean +/- s.d. Statistical analysis was performed by a two-sided ratio paired t-test for e , f, i and unpaired two-sided Student’s t-test for j-l.

Journal: bioRxiv

Article Title: Spatial patterning of fibroblast TGFβ signaling underlies treatment resistance in rheumatoid arthritis

doi: 10.1101/2025.03.14.642821

Figure Lengend Snippet: a-b, Schematic of experimental workflow and UMAP plot of isolated single cells from the indicated micromass organoid conditions (ref ). Fibroblast Monoculture: organoid containing fibroblasts only; Fibroblasts + ECs: organoids containing fibroblasts and ECs; Fibroblasts + ECs + DAPT: organoids containing fibroblasts and ECs treated with a NOTCH inhibitor. c, UMAP plot of cells from organoid culture shaded by level of fibrogenic gene signature score as calculated with UCell. d, Heatmap of selected genes grouped by experimental condition. e-f, RT-qPCR analysis of TGFB isoform and receptor gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without DAPT (10 uM) for 72 hours. g-h, Immunoblots of TGFβ isoforms and receptors with lysates from unstimulated or DLL4 stimulated fibroblasts (72h). i, RT-qPCR analysis of COL1A1 , COL3A1 and COL6A1 gene expression on unstimulated or DLL4 stimulated fibroblasts treated with or without TGFβ inhibitor (SB431542; 10 uM) or DAPT (10 uM) for 72h. j , ELISA quantification of fibroblast production of pro-collagen I alpha 1 over 24h after 5 days of treatment with siRNA (20nM) during or without DLL4 stimulation. k-l , RT-qPCR and ELISA quantification of POSTN (k) and COMP (l) after 72 hours of stimulation with recombinant TGFβ1 (10 ng ml−1) (left) or with DLL4 stimulation and siRNA targeting of TGFBR2 (right). For e,f,i each data point represents an independent cell line (n = 6) and for j-l each data point represents biological replicates (n = 3) from a single cell line and are representative of at least two independent experiments. Data are shown as mean +/- s.d. Statistical analysis was performed by a two-sided ratio paired t-test for e , f, i and unpaired two-sided Student’s t-test for j-l.

Article Snippet: In some conditions, cells were treated with TGF beta-1 (Peprotech, 10 ng ml−1), DAPT (Selleckchem, 10 uM) or TGFβ inhibitor (SB431542).

Techniques: Isolation, Quantitative RT-PCR, Gene Expression, Western Blot, Enzyme-linked Immunosorbent Assay, Recombinant